Dr. Qiuhong Wang

Assistant Professor

Food Animal Health Research Program

Lab 137
OARDC Wooster, OH 44691
Phone: 330-263-3960
Fax: 330-263-3677
Email: wang.655@osu.edu

Biographical Information

    • Bachelor of Medicine (M.D. equivalent), Beijing Medical University, China
    • MS, University of Tokyo
    • Ph.D, The Ohio State University
    • Post-doc, Medical College of Wisconsin
    • Research Scientist, The Ohio State University

Research Area

My current research focus is on enteric caliciviruses and coronaviruses, including diagnosis of viral infections, molecular epidemiology, molecular characterization of new viruses, propagation of enteric viruses in cell culture, molecular mechanisms of cell culture adaptation and attenuation, interspecies transmission of viruses between humans and animals, the mechanism of enteric virus transmission through leafy greens, and the development of vaccines.

Selected Publications

Publications

  1. Oka T, Stoltzfus GT, Zhu C, Jung K, Wang Q, and Saif LJ.  Attempts to grow human noroviruses, a sapovirus and animal noroviruses in vitro. Plos One. (Accepted on May 17, 2017)
  2. Hou Y, Lin CM, Yokoyama M, Yount BL, Marthaler D, Douglas AL, Ghimire S, Qin Y, Baric RS, Saif LJ, and Wang Q. Deletion of a 197 amino acid-region in the N-terminal domain of spike protein attenuates porcine epidemic diarrhea virus. J Virol. 91 (14).
  3. Lin CM, Hou Y, Marthaler D, Gao X, Liu X, Zhang L, Saif LJ, Wang Q. Attenuation of an Original US Porcine Epidemic Virus Strain PC22A via Serial Cell Culture Passage. Veterinary Microbiology. 2017; 201:62-71.
  4. Lin CM, Saif LJ, Marthaler D, Wang Q. Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains. Virus research. 2016.(Review)
  5. Liu X, Wang Q. Reverse transcription-PCR assays for the differentiation of various US porcine epidemic diarrhea virus strains. J Virol Methods. 2016 Aug;234:137-41. doi: 10.1016/j.jviromet.2016.04.018. Epub 2016 Apr 28.
  6. Oka T, Lu Z, Phan T, Delwart EL, Saif LJ, Wang Q. Genetic Characterization and Classification of Human and Animal Sapoviruses. PloS one. 2016; 11(5):e0156373.
  7. Beall A, Yount B, Lin CM, Hou Y, Wang Q, Saif LJ, Baric R. Characterization of a Pathogenic Full-Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A. mBio. 2016; 7(1):e01451-15.
  8. Gao X, Esseili MA, Lu Z, Saif LJ, Wang Q. Recognition of Histo-Blood Group Antigen-Like Carbohydrates in Lettuce by Human GII.4 Norovirus. Applied and environmental microbiology. 2016; 82(10):2966-74.
  9. Oka T, Wang Q, Katayama K, Saif LJ. Comprehensive review of human sapoviruses. Clinical microbiology reviews. 2015; 28(1):32-53. (Review)
  10. Lin CM, Gao X, Oka T, Vlasova AN, Esseili MA, Wang Q, Saif LJ. Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains. Journal of virology. 2015; 89(6):3332-42.
  11. Esseili MA, Chin A, Saif LJ, Miller SA, Qu F, Lewis Ivey ML, Wang Q. Postharvest survival of porcine sapovirus, a human norovirus surrogate, on phytopathogen infected leafy greens. Journal of food protection. 2015; 78:1472-80.
  12. Hu H, Jung K, Vlasova AN, Chepngeno J, Lu Z, Wang Q, Saif LJ. Isolation and characterization of porcine deltacoronavirus from pigs with diarrhea in the United States. Journal of clinical microbiology. 2015; 53(5):1537-48.
  13. Esseili MA, Saif LJ, Farkas T, Wang Q. Feline Calicivirus, Murine Norovirus, Porcine Sapovirus, and Tulane Virus Survival on Postharvest Lettuce. Applied and environmental microbiology. 2015; 81(15):5085-92.
  14. Liu X, Lin CM, Annamalai T, Gao X, Lu Z, Esseili MA, Jung K, El-Tholoth M, Saif LJ, Wang Q. Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain. Veterinary research. 2015; 46:109.
  15. Liu X, Oka T, Wang Q. Genomic characterization of a US porcine kobuvirus strain. Archives of microbiology. 2015; 197(8):1033-40.
  16. Esseili MA, Gao X, Tegtmeier S, Saif LJ, Wang Q. Abiotic Stress and Phyllosphere Bacteria Influence the Survival of Human Norovirus and Its Surrogates on Preharvest Leafy Greens. Applied and environmental microbiology. 2015; 82(1):352-63.
  17. Lu Z, Yokoyama M, Chen N, Oka T, Jung K, Chang KO, Annamalai T, Wang Q, Saif LJ. Mechanism of Cell Culture Adaptation of an Enteric Calicivirus, the Porcine Sapovirus Cowden Strain. Journal of virology. 2015; 90(3):1345-58.
  18. Lin CM, Annamalai T, Liu X, Gao X, Lu Z, El-Tholoth M, Hu H, Saif LJ, Wang Q. Experimental infection of a US spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original US PEDV infection. Veterinary research. 2015; 46:134.
  19. Thomas C, Jung K, Han MG, Hoet A, Scheuer K, Wang Q, Saif LJ. Retrospective serosurveillance of bovine norovirus (GIII.2) and nebovirus in cattle from selected feedlots and a veal calf farm in 1999 to 2001 in the United States. Archives of virology. 2014; 159(1):83-90.
  20. Marion JW, Lee C, Lee CS, Wang Q, Lemeshow S, Buckley TJ, Saif LJ, Lee J. Integrating bacterial and viral water quality assessment to predict swimming-associated illness at a freshwater beach: a cohort study. PloS one. 2014; 9(11):e112029.
  21. Jung K, Scheuer KA, Zhang Z, Wang Q, Saif LJ. Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves. Veterinary microbiology. 2014; 168(1):202-7. NIHMSID: NIHMS541856.
  22. Takanashi S, Saif LJ, Hughes JH, Meulia T, Jung K, Scheuer KA, Wang Q. Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures. Archives of virology. 2014; 159(2):257-66. NIHMSID: NIHMS518636.
  23. Lee CS, Lee C, Marion J, Wang Q, Saif L, Lee J. Occurrence of human enteric viruses at freshwater beaches during swimming season and its link to water inflow. The Science of the total environment. 2014; 472:757-66.
  24. May NA, Wang Q, Balbo A, Konrad SL, Buchli R, Hildebrand WH, Schuck P, Hudson AW. Human herpesvirus 7 U21 tetramerizes to associate with class I major histocompatibility complex molecules. Journal of virology. 2014; 88(6):3298-308.
  25. Jung K, Wang Q, Scheuer KA, Lu Z, Zhang Y, Saif LJ. Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs. Emerging infectious diseases. 2014; 20(4):662-5.
  26. Oka T, Saif LJ, Yang Z, Scheuer KA, Stoltzfus GT, Wang Q. First Complete Genome Sequences of Genogroup VI Porcine Sapoviruses. Genome announcements. 2014; 2(2).
  27. Amimo JO, Okoth E, Junga JO, Ogara WO, Njahira MN, Wang Q, Vlasova AN, Saif LJ, Djikeng A. Molecular detection and genetic characterization of kobuviruses and astroviruses in asymptomatic local pigs in East Africa. Archives of virology. 2014; 159(6):1313-9. NIHMSID: NIHMS593495
  28. Cromeans T, Park GW, Costantini V, Lee D, Wang Q, Farkas T, Lee A, Vinjé J.
    Comprehensive comparison of cultivable norovirus surrogates in response to different inactivation and disinfection treatments. Applied and environmental microbiology. 2014; 80(18):5743-51.
  29. Vlasova AN, Marthaler D, Wang Q, Culhane MR, Rossow KD, Rovira A, Collins J, Saif LJ. Distinct characteristics and complex evolution of PEDV strains, North America, May 2013-February 2014. Emerging infectious diseases. 2014; 20(10):1620-8.
  30. Oka T, Saif LJ, Marthaler D, Esseili MA, Meulia T, Lin CM, Vlasova AN, Jung K, Zhang Y, Wang Q. Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene. Veterinary microbiology. 2014; 173(3-4):258-69.
  31. Iizuka S, Takai-Todaka R, Ohshiro H, Kitajima M, Wang Q, Saif LJ, Wakita T, Noda M, Katayama K, Oka T. Detection of multiple human sapoviruses from imported frozen individual clams. Food and environmental virology. 2013; 5(2):119-25.
  32. Scheuer KA, Oka T, Hoet AE, Gebreyes WA, Molla BZ, Saif LJ, Wang Q. Prevalence of porcine noroviruses, molecular characterization of emerging porcine sapoviruses from finisher swine in the United States, and unified classification scheme for sapoviruses. Journal of clinical microbiology. 2013; 51(7):2344-53.

Research Funding

NIFA, USDA. Molecular attenuation mechanisms of porcine epidemic diarrhea virus in pigs.

PI: Wang, Q, Co-PI: Saif, LJ; Co-Is: Baric, R, Sims A, and Jung, K

Goal: To exploit different strategies to generate PEDV strains with reduced virulence, to identify genomic hot spots related to attenuation, and finally to verify those hot spots using infectious clone platform.  Our final goal is to generate and identify PEDV vaccine candidates, and obtain knowledge to advance the development of a safe attenuated PEDV vaccine to prevent further deadly PEDV outbreaks in the US, and to aid in innovative vaccine design against other fatal animal and human coronavirus diseases, such as Severe Acute Respiratory Syndrome (SARS) and Middle East respiratory syndrome (MERS).

 

NIFA, USDA. Human noroviruses: Mechanisms of attachment and internalization in leafy greens. PI: Wang, Q; Co-Is: Saif, LJ, Esseili, MA, Ivey, M, Meulia,T

Goal: To identify the capsid amino acids important in norovirus binding to lettuce leaves, to identify the lettuce cell wall carbohydrate(s) involved in norovirus binding and plant-derived lectin inhibitors for binding, to investigate whether specific binding of norovirus to lettuce carbohydrates enhances virus persistence and entry into the leaf, and  to elucidate mechanisms of norovirus (and infectious sapovirus) entry through the roots, their interaction with components of the vascular system, and their internal transport inside the lettuce plant. Our final goal is to improve public health by decreasing gastroenteritis outbreaks/cases due to leafy green contamination of human enteric noroviruses and sapoviruses.