Dr. Renukaradhya Gourapura (Aradhya)

Associate Professor

Food Animal Health Research Program
Veterinary Preventive Medicine
The Ohio State University
1680 Madison Avenue
Wooster, OH 44691
Office: 330-263-3748
Lab: 330-263-3618
Fax: 330-263-3677
Email: gourapura.1@osu.edu

http://www.oardc.ohio-state.edu/fahrp/pageview.asp?id=836

http://cmib.osu.edu/people/faculty/renukaradhyagourapura/

http://vet.osu.edu/RenukaradhyaGourapura

Biographical Information

  • BVSc (DVM), 1991, University of Agricultural Sciences, Bangalore, India
  • MVSc (MS), 1994, University of Agricultural Sciences, Bangalore, India
  • PhD, 2002, Indian Institute of Science, Bangalore, India
  • Postdoc, 2002-2008, Dept. of Microbiology & Immunology, Indiana University School of Medicine, Indianapolis, IN, USA

Research Area

My laboratory research focus is on mucosal immunology of food animals infected with infectious and zoonotic diseases. Unlike systemic delivery, mucosal delivery of potent vaccines is gaining increased attention to increase the breadth of cross-protective immunity against infections which cause severe disease at mucosal sites. We are interested to understand mucosal immune responses in pigs and other species of food animals to viral and bacterial infections, and to vaccines delivered directly to mucosal sites. Currently, we are evaluating potent bacterial-based and synthetic adjuvants, which were proven effective and safe in other species of animals to use in food animals. Our ongoing studies are aimed to potentiate the cross-protective efficacy of modified live virus and biodegradable nanoparticle-entrapped inactivated virus vaccines of porcine reproductive and respiratory syndrome (PRRS) virus and influenza virus, coadministered intranasally with a potent adjuvant to pigs. Other focus of our study is to understand host-pathogen interactions, viral pathogenesis, and immune evasion mechanisms in food animals infected with viral and bacterial pathogens; and developing a pig model for biomedical research.

Selected Publications

2016 and 2017

  1. Ouyang K, J. Hiremath, B. Binjawadagi, D-L. Shyu, S. Dhakal, J. Arcos, R. Schleappi, L. Holman, M. Roof, J.B. Torrelles, and G.J. Renukaradhya* (2016). Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge study. Veterinary Research, 47(1):45. PMID: 26988085
  2. Dwivedi V, C. Manickam, S. Dhakal, B. Binjawadagi, K. Oyuang, J. Hiremath, M. Khatri, J. Gervey-Hague, C-W. Lee, and G.J. Renukaradhya* (2016). Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs. Veterinary Microbiology, 186:157-163. PMID: 270167703.
  3. Hiremath J, K. Kyung-il, M. Elaish, B. Binjawadagi, K. Ouyang, S. Dhakal, J.B. Torrelles, C.W. Lee, and G.J. Renukaradhya* (2016). Entrapment of H1N1 influenza virus derived conserved peptides in PLGA nanoparticles enhances T cell response and vaccine efficacy in pigs. PLoS One, 11(4):e0151922. PMID: 27093541
  4. Binjawadagi B, S.L. Yashavanth, Z. Longchao, S. Dhakal, J. Hiremath, K. Ouyang, D-L. Shyu, J. Arcos, A. Gilbertie, F. Zuckermann, J.B. Torrelles, D. Jackwood, Y. Fang, and G.J. Renukaradhya* (2016). Development of porcine reproductive and respiratory syndrome virus-like-particle and evaluation of its immunogenicity in pigs. Archives of Virology, 161(6):1579-1589. PMID: 27008569
  5. Tabynov, K, A. Sansyzbay, Z. Tulemissova, S. Dhakal, G.J. Renukaradhya, and Mambetaliyev, M (2016). Inactivated porcine reproductive and respiratory syndrome virus vaccine adjuvanted with MontanideTM Gel 01 ST elicits virus-specific cross-protective inter-genotypic response in piglets. Vet Microbiol. 192:81-89. PMID: 27527768
  6. Tabynov, K, M. Orynbayev, G.J. Renukaradhya and A. Sansyzbay (2016). Simultaneous subcutaneous and conjunctival administration of the influenza viral vector Brucella abortus vaccine to pregnant heifers provides better protection against B. abortus 544 infection than the commercial B. abortus S19 vaccine. Vaccine, 34(42):5049-52. PMID: 27595898
  7. Tabynov, K, B. Yespembetov, N. Matikhan, S. Ryskeldinova, N. Zinina, Z. Kydyrbayev, N. Assanzhanova, K. Tabynov, G.J. Renukaradhya and A. Sansyzbay (2016). First evaluation of an influenza viral vector based Brucella abortus vaccine in sheep and goats: assessment of safety, immunogenicity and protective efficacy against Brucella melitensis infection. Vet Microbiol. 197; 15-20. PMID: 27938677
  8. Dhakal, S, J. Hiremath, K. Bondra, Y.S. Lakshmanappa, D. Shyu, K. Oyuang, K. Kang, B. Binjawadagi, J. Goodman, K. Tabynov, S. Krakowka, B. Narasimhan, C.W. Lee and G.J. Renukaradhya (2017). Biodegradable nanoparticle delivery of inactivated swine influenza virus vaccine provides heterologous cell-mediated immune response in pigs. J Control Release, 247:194-205. PMID: 28057521
  9. Dhakal, S, J. Goodman, K. Bondra, Y.S. Lakshmanappa, J. Hiremath, D. Shyu, K. Oyuang, K. Kang, S. Krakowka, M.J. Wannemuehler, C.W. Lee, B. Narasimhan and G.J. Renukaradhya (2017). Polyanhydride nanovaccine against swine influenza virus in pigs. Vaccine, 35(8): 1124-1131. PMID: 28117173
  10. Elaish, M. A. Ali, K. Kang, M. Xia, M. Ibrahim, J.M. Ngunjiri, H. Jang, J. Hiremath, S. Dhakal, X. Jiang, G.J. Renukaradhya, and C.W. Lee (2017). Supplementation of inactivated influenza vaccine with the norovirus P particle-M2e chimeric vaccine enhances the protection against heterologous virus challenge in chickens. Plos One, 12(2):e0171174. PMID: 28151964

2015

 

  1. Yanhua L, D-L Shyu, P. Shang, J. Bai, K. Ouyang, S. Dhakal, J. Hiremath, B. Binjawadagi, G.J. Renukaradhya*, Y. Fang* (2015). Mutations in a highly conserved motif of nsp1β protein attenuate the innate immune suppression function of porcine reproductive and respiratory syndrome virus (PRRSV). Journal of Virology, 90(7):3584-3599. PMID: 267927334
  2. Ouyang K, D-L. Shyu, S. Dhakal, J. Hiremath, B. Binjawadagi, S.L. Yashavanth, R. Guo, R. Ransburgh, K.M. Bondra, P. Gauger, J. Zhang, T. Specht, A. Gilbertie, W. Minton, Y. Fang, and G.J. Renukaradhya* (2015). Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under field conditions. Veterinary Research, 46(1):140. PMID: 266672293
  3. Thomas M, Z. Wang, C.C. Sreenivasan, B.M. Hause, G.J. Renukaradhya, F. Li, D.H. Francis, R.S. Kaushik and M. Khatri (2015). Poly I:C adjuvanted inactivated swine influenza vaccine induces heterologous protective immunity in pigs. Vaccine, 33(4):542-548. PMID: 25437101
  4. Renukaradhya G.J., X-J, Meng, J.G. Calvert, M. Roof, K.M. Lager (2015). Inactivated and subunit vaccines against porcine reproductive and respiratory syndrome: Current status and future direction. Vaccine, 33(27):3065-3072. PMID: 25980425.
  5. Renukaradhya G.J., X-J, Meng, J.G. Calvert, M. Roof, and K.M. Lager (2015). Live porcine reproductive and respiratory syndrome virus-based vaccines: current status and future direction. Vaccine, 33(33):4069-4080. PMID: 26148878
  6. Renukaradhya, G.J., B, Narasimhan, S.K. Mallapragada (2015). Respiratory nanoparticle-based vaccines and challenges associated with animal models and translation. Journal of Control Release, 219:622-631. PMID: 26410807
  7. Lunney J.K., Y. Fang, A. Ladinig, N. Chen, Y. Li, B. Rowland, G.J. Renukaradhya (2015). Porcine reproductive and respiratory syndrome virus (PRRSV): Pathogenesis and Interaction with the Immune System. Annual Review of Animal Biosciences, 14:25. PMID: 26646630

Other Important Publications

  1. Jung, K., G.J. Renukaradhya, K.P. Alekseev, Y. Fang, Y. Tang, and L.J. Saif (2009). Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J. Gen. Virol., 90:2713-2723. PMID: 19656969
  2. Jung, K., A. Gurnani, G.J. Renukaradhya, and L.J. Saif (2010). Nitric oxide is elicited and inhibits viral replication in pigs infected with porcine respiratory coronavirus but not porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopath., 136:335-339. PMID: 20409593.
  3. Renukaradhya, G.J.*, K.P. Alekseev, K. Jung, Y. Fang and L.J. Saif (2010). Porcine reproductive and respiratory syndrome virus induced immunosuppression exacerbate the inflammatory response to porcine respiratory coronavirus in pigs. Viral Immunol. 23(5):457-466. PMID: 20883160
  4. Khatri, M., V. Dwivedi, S. Krakowka, C. Manickam, A. Ali, L. Wang, Z. Qin, G.J. Renukaradhya* and Chang-Won Lee* (2010). Swine influenza virus induces acute inflammatory immune responses in pig lungs: a potential animal model to understand human pandemic H1N1 influenza virus. J. Virol. 84(21):11210-11218. PMID: 20719941
  5. Renukaradhya, G.J.*, C. Manickam, M. Khatri, A. Rauf, X. Li, M. Tsuji, G. Rajashekara, and Varun Dwivedi (2011). Functional invariant NKT cells in pig lungs regulate airway hyperreactivity: a potential animal model. J. Clin. Immunol. 31(2):228-39. PMID: 21042929
  6. Khandelwal, A., G.J. Renukaradhya, M. Rajasekhar, G. Lakshmi Sita, and M. S. Shaila (2011). Immune responses to Hemagglutinin-Neuraminidase protein of peste des petits ruminants virus expressed in transgenic peanut plants in sheep. Vet. Immunol. Immunopathol. 140(3-4):291-296. PMID: 21211855
  7. Dwivedi V, C. Manickam, R. Patterson, K. Dodson, and G.J. Renukaradhya* (2011). Intranasal delivery of whole cell lysate of Mycobacterium tuberculosis induces protective immune responses to a modified live porcine reproductive and respiratory syndrome virus vaccine in pigs. Vaccine 29(23):4067-76. PMID: 21419164
  8. Dwivedi V, C. Manickam, R. Patterson, K. Dodson, M. Murtaugh, J.B. Torrelles, L.S. Schlesinger, and G.J. Renukaradhya* (2011). Cross-protective immunity to porcine reproductive and respiratory syndrome virus by intranasal delivery of a live virus vaccine with a potent adjuvant. Vaccine 29(23):4058-66. PMID: 21419162
  9. Binjawadagi B, V. Dwivedi, C. Manickam, J.B. Torrelles, and G.J. Renukaradhya* (2011). Intranasal delivery of an adjuvanted modified live porcine reproductive and respiratory syndrome virus vaccine reduces the ROS production. Viral Immunol. 24(6):1-8. PMID: 22111597
  10. Dwivedi V, C. Manickam, B. Binjawadagi, R. Patterson, D. Linhares, M.P. Murtaugh, and G.J. Renukaradhya* (2012). Evaluation of immune responses to porcine reproductive and respiratory syndrome virus in pigs during early stage of infection. Virology Journal. 9:45: 1-9. PMID: 22340040
  11. Broxmeyer H.E, K. Christopherson, G. Hangoc, S. Cooper, C. Mantel, G.J. Renukaradhya*, and Brutkiewicz R.R (2012). CD1d Expression on and regulation of murine hematopoietic stem and progenitor cells. Blood, 119(24): 5731-41. PMID: 22535665
  12. Dwivedi V, C. Manickam, B. Binjawadagi, Joyappa, D, and G.J. Renukaradhya* (2012). Biodegradable nanoparticle-entrapped vaccine induces cross-protective immune response against a virulent heterologous respiratory viral infection in pigs. PLoS One. 7(12):e51794. PMID: 23240064
  13. Manickam C, V. Dwivedi, R. Patterson, K. Dodson and G.J. Renukaradhya* (2013). Porcine reproductive and respiratory syndrome virus induces pronounced immune modulatory responses at mucosal tissues in the parental vaccine strain VR2332 infected pigs. Vet. Microbiol., 162(1):68-77. PMID: 22995873
  14. Manickam C, V. Dwivedi, J. Miller, T. Papenfuss and G.J. Renukaradhya* (2013) Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs. Viral Immunol., 26(1):102-108. PMID: 23308386
  15. Annamalai T, R. Pina-Mimbela, A. Kumar, B. Binjawadagi, G.J. Renukaradhya and G. Rajashekara (2013). Evaluation of nanoparticle encapsulated OMPs for the control of Campylobacter jejuni colonization in chickens. Poultry Science, 92:2201-2211. PMID:23873570
  16. Dwivedi V, C. Manickam, B. Binjawadagi, and G.J. Renukaradhya* (2013). PLGA nanoparticle entrapped killed porcine reproductive and respiratory syndrome virus vaccine helps in viral clearance in pigs. Vet. Microbiol., 166(1-2):47-58. PMID:23764272
  17. Ouyang K, B. Binjawadagi, A. Kittawornrat, C. Olsen, J. Hiremath, N. Elkalifa, R. Schleappi, J. Wu, J. Zimmerman, G.J. Renukaradhya.* (2013). Development and Validation of an Assay to detect Porcine Reproductive and Respiratory Syndrome Virus specific Neutralizing Antibody Titers in Pig Oral Fluid Samples. Clinical and Vaccine Immunol., 20(8):1305-1313. PMID: 23784856
  18. Renukaradhya G.J, M.A. Khan, R.M. Gallo, D. Shaji, J. Liu, R.R. Brutkiewicz (2013). Forming a complex with MHC class I molecules interferes with mouse CD1d functional expression. PLoS One. 8(8):e72867. PMID: 24009709
  19. Olsen C, L. Karriker, C. Wang, G.J. Renukaradhya, B. Binjawadagi, A. Kittawornrat, S. Lizano, J. Coetzee, R. Main, A. Meiszberg, Y. Panyasing, J. Zimmerman (2013). Effect of collection material on pig oral fluid testing results. Veterinary Journal, 198(1):158-163. PMID: 24011474
  20. Binjawadagi B, V. Dwivedi, C. Manickam, K. Ouyang, Y. Wu, J. Lee, J.B. Torrelles and G.J. Renukaradhya* (2014). Adjuvanted nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits better cross-protective response in pigs. International Journal of Nanomedicine, 9:679-694. PMID: 24493925
  21. Ouyang K, S.A. Woodiga, V. Dwivedi, C.M. Buckwalter, A.K. Singh, B. Binjawadagi, J. Hiremath, C. Manickam, R. Schleappi, M. Khatri, J. Wu, S.J. King, and G.J. Renukaradhya* (2014). Pretreatment of epithelial cells with live Streptococcus pneumoniae has no detectable effect on influenza A virus replication in vitro. PLoS One, 9(3):e90066. PMID: 24594847
  22. Binjawadagi B, V. Dwivedi, C. Manickam, K. Ouyang, J.B. Torrelles and G.J. Renukaradhya* (2014). An innovative approach to induce cross-protective immunity against porcine reproductive and respiratory syndrome virus in the lungs of pigs through adjuvanted nanotechnology based vaccination. International Journal of Nanomedicine, 9:1519-1535. PMID: 24711701
  23. Bailey J, I. Abhirami, G.J. Renukaradhya, L. Yinling, N. Hoa, R.R. Brutkiewicz (2014). Inhibition of CD1d-mediated Antigen Presentation by the TGF-β/Smad Signaling Pathway. Immunology, 143(4):679-691. PMID: 24990409